Technologies

CCR Genomics Core

The CCR Genomics Core provides NCI and NIH scientific communities with rapid and flexible access to modern genomic technologies. The Core is an “open access” facility with the goal of providing efficiency and quality of a centralized facility with the speed and convenience of dedicated laboratory instrumentation and experienced staff. Training and extensive guidance in cutting edge techniques are provided to a very broad spectrum of users, who would have otherwise been unable to incorporate these advanced methodologies in their research. Working closely with the Office of Science and Technology Resources (OSTR), the Core helps to identify, evaluate and make available new technologies as they emerge to CCR scientific community. The Core also provides support to the new Single-Cell Analysis Facility (SCAF) with its one-stop single cell isolation, sequencing, and analysis service in a joint effort with the Sequencing Facility & Genomics Technology Core in Frederick.

For general information about the CCR Genomics Core or to inquire about accessing the technologies please contact us at the group email ncilecdnacore@mail.nih.gov. To register to use instruments or to request services go to the CCR Genomics Core iLab webpage https://nci.corefacilities.org/account/login

Technologies

Next Generation Sequencing

iSeq, MiSeq,3-NextSeq 2000

Digital Gene Expression / Nanostring nCounter System

Robust method for multiplexing up to 800 gene expression targets

Digital Droplet PCR

(Bio-Rad QX200 ddPCR system)

Analytical and Preparative Electrophoresis

Agilent Tapestations 4150 and 4200, Pippin HT

NanoString GeoMX DSP

Whole-tissue spatial multiomics, including whole transcriptome and 570+ proteins

Long-Read Sequencing

Oxford Nanopore Technologies Mk1C, GridION, PromethION2Solo

Common Library Preparation Protocols Used by the Core

Below is a list of standard library preparation protocols utilized by the Core facility. If you have questions or need assistance selecting a protocol, contact the Core staff at ncilecdnacore@mail.nih.gov.

Illumina Protocols

ChIP-Seq

Chromatin immunoprecipitation followed by sequencing to profile protein–DNA interactions.

RNA-Seq (polyA selection or ribodepletion)

Transcriptome profiling; choose polyA or ribodepletion depending on your sample.

Illumina Nextera XT

Rapid library prep for small genomes (bacteria, viruses, etc.).

ONT Protocols

GridION rapid sequencing (v14, plasmid)

Fast prep for plasmid sequencing on GridION.

PromethION ligation (gDNA, native)

High-throughput long-read ligation prep for genomic DNA.

GridION ligation (gDNA, native)

Ligation-based long-read prep tailored for GridION flow cells.

PromethION ligation (amplicons, native)

Optimized amplicon prep for PromethION long-read sequencing.

GridION ligation (amplicons, native)

Amplicon sequencing workflow on GridION devices.