Next-Generation Sequencing (NGS)

Sequencing is available for pre-made, ready-to-sequence libraries. Library preparation services (Bulk RNA-seq and ChIP-seq) may be offered on a limited basis for non-routine samples (e.g., human-derived or non-renewable samples). Such requests are subject to additional review and approval by the core facility and institutional leadership.

Sample Quality Control

Quality assessment of RNA and DNA samples is performed using TapeStation assays (see attached documentation).

Instrumentation and Capacity

Sequencing capacity is limited to bench top instruments, including one (1) MiSeq and four (4) NextSeq 2000 systems.

Pricing Models

The Core operates on a per-run, per-flowcell pricing model and does not split samples across flow cells. Each sequencing run occupies a single flow cell, and the customer is charged the full cost of the Illumina sequencing kit. Pricing for the sequencing includes charges for quality control (QC) of the pooled sample (1) and the flow cell/run. Non-NCI customers are assessed an additional surcharge. Volume discounts or institute-level arrangements are not currently offered (see attached information).

Turnaround Time

The typical turnaround time for samples that are ready to sequence and have passed quality control is approximately one (1) week, contingent upon the availability of the required sequencing kits.

Data Delivery and Analysis

Sequencing data will be delivered via a secure download link (FASTQ or BCL files only). Data are stored on core servers for two years, after which users are responsible for long-term data storage. The core facility does not currently provide secondary analysis or comprehensive bioinformatics support.

Long-Read Sequencing Capabilities

Long-read sequencing is supported using the Oxford Nanopore GridION and P2 Solo platforms. The GridION supports up to five (5) MinION flow cells per run, while the P2 Solo supports up to two (2) PromethION flow cells per run.

Experience supporting human samples and relevant compliance considerations

The Core is not CLIA-certified and therefore does not perform clinical testing or return results for clinical use. Appropriate institutional and regulatory requirements (e.g., IRB approval and informed consent, when applicable) are the responsibility of the submitting investigator.