Supported by CCR Office of Science and Technology Resources (OSTR)

Whole Plasmid Sequencing

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The CCR Genomics Core has developed a low-cost approach for complete plasmid sequencing using Oxford Nanopore sequencing technology in combination with automated sequence analysis. This service offers many advantages over conventional primer walking and current NGS methods, including lower cost and faster turnaround time.

Service Features
• Fast turnaround time
• Cost-effective
• Complete characterization of unknown plasmids
• Complete validation of known plasmids
• Independent of sequencing primers (no primers required)

For detailed sample preparation and submission requirements, please contact us.

Plasmid QC APP

This DNAnexus application implements a QC evaluation module as part of the larger application, which is based on the nf-core workflow, wf-clone-validation, developed by Oxford Nanopore for De novo assembly of plasmids. This module should help you evaluate the accuracy and reliability of the assemblies generated by this pipeline.

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