Supported by CCR Office of Science and Technology Resources (OSTR)

Sanger Sequencing

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What is Sanger Sequencing?

Sanger sequencing is the gold standard for sequencing technology in that it provides a high degree of accuracy, long-read capabilities, and the flexibility to support a diverse range of applications. Sequencing can be performed on a variety of templates including PCR products, plasmids and as well as bacterial genomic DNA, BACs and cosmids.

Reading the Sequence

Dye-terminator sequencing is performed using a modified protocol supplied by Applied Biosystems BigDye Terminator 1.1 cycle sequencing kits and analyzed on either a 24-capillary 3500XL.