Next-Generation Sequencing (NGS)

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Available Instruments: MiSeq System & NextSeq 550 System

Next-Generation Sequencing (NGS) Services

The CCR Genomics Core utilizes Illumina platforms for all NGS services. The NextSeq 550 Sequencing System is a fast, flexible, high-throughput benchtop sequencer with exceptional data accuracy. It is used for projects such as exome sequencing, ChIP-Seq and RNA-Seq. The NextSeq 550 System also has the ability to scan microarrays including the Infinium MethylationEPIC BeadChip and other select BeadChips.

MiSeq is the smaller scale sequencer which provides an ideal platform for rapid turnaround. MiSeq is also a cost-effective tool for various analyses focused on targeted gene sequencing (amplicon sequencing and target enrichment), metagenomics, and gene expression studies.

Library preparation by the user is accepted but the core does offer library preparation services (contact the Core for details). Core personnel assess library quality, perform sequencing, assess data quality. Data will be delivered to core users via presigned Cleversafe URLs. Front-end support includes bioinformatic consultation, assistance with experimental design and advice on library preparation.

Sequencer Comparison