Next-Generation Sequencing (NGS)

Next-Generation Sequencing (NGS) Services

The CCR Genomics Core provides Next-Generation Sequencing (NGS) services using Illumina sequencing technology. The Core facility has multiple Illumina desktop sequencers to include the iSeq, MiSeq, and (4) NextSeq 2000’s. Each sequencing platform delivers exceptional data quality and performance, with flexible throughput and simple, streamlined workflows. Applications include targeted gene sequencing (amplicon sequencing and target enrichment), metagenomics, gene expression studies, ChIP-Seq and RNA-Seq. The NextSeq 550 also has the ability to scan microarrays including the Infinium MethylationEPIC BeadChip and other select BeadChips.

MiSeq is the smaller scale sequencer which provides an ideal platform for rapid turnaround. MiSeq is also a cost-effective tool for various analyses focused on targeted gene sequencing (amplicon sequencing and target enrichment), metagenomics, and gene expression studies.

Core personnel provide a full spectrum of experimental support. From bioinformatic consultation, experimental design assistance and advice on library preparation to library quality assessment and preparation, sequencing, and data analysis. Libraries may be prepared either by the Core or their customers. Data will be delivered to Core users via presigned Cleversafe URLs. Full bioinformatics support is provided on a case-by-case basis.